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Home -> Gene Analysis -> Telomerase -> Quantitative PCR Kit
Lysis Buffer for TeloExpress Kit
| Cat.-#: |
XT-103 |
| Quantity: |
10ml |
| Price: |
$139 |
| Storage: |
+4C |
| Shipment: |
-70 C |
| Note: |
For Research Use Only
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| Telomerase |
The TeloExpress quantitative telomerase detection kit is an optimized and validated real-time PCR Kit for the quantification of human telomerase activity that can be utilized in most real-time PCR platforms. In this telomeric repeat amplification protocol (TRAP), the telomerase activity in cell extracts adds the telomeric (TTAGGG) repeat sequences to the 3?-end of an oligonucleotide substrate. The number of repeat sequences added by the enzyme is quantified using real-time PCR by measuring the increase in SYBR green fluorescence upon binding to DNA. Real-time PCR is conducted under conditions in which primer dimer formation is negligible and the extent of repeat amplification is directly proportional to telomerase activity. The TeloExpress kit shows linear responses to input telomerase enzyme and can predict input telomerase activity with an error of less than 20%.
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Product Manual